Emergencies often occur at night or during adverse weather conditions. This chapter provides an overview on the importance of integrating six key variables to achieve operational safety in medical emergency transportation systems and how it can be generalized to general acute health care situations for elders. They are the organizational culture, situation awareness, its safety culture, safety management, risk management, and training. In the case of pilots flying medical emergency helicopters, organizational culture may influence the crew’s perception of the critical factors in their immediate environment that informs their situation awareness. Several types of stress factors can exist in the medical emergency transportation environment, as well as in other senior-citizen-focused health care. Health care practitioners must be familiar with the safety regulations that govern the operations of medical emergency systems in their communities or regions.