Purpose: To assess the levels and prevalence of depression, anxiety, and stress and to identify factors associated with these emotions among Jordanian patients with type 2 diabetes mellitus. Methods: This descriptive study recruited 149 participants through a convenience sampling technique. Depression, anxiety, and stress burdens were measured through Arabic short version of Depression, Anxiety, and Stress Scale (DASS-21; Lovibond & Lovibond, 1995). Results: There were 28.8%, 49.5%, and 33.5% of participants who had moderate-to-extremely severe levels of depression, anxiety, and stress, respectively. The depression, anxiety, and stress burdens were associated with patients’ gender and educational level, and the highest level of depression, anxiety, and stress burdens levels were associated with the presence of diabetes complications and other chronic illness. Conclusion: The findings from this study can guide the health providers to address psychological status for patients with type 2 diabetes mellitus in their care plans.