For new neurophysiologic intraoperative monitoring (NIOM) staff, the operating room can be a very intimidating environment. Becoming familiar with various operating room personnel, equipment and workflow, as well as understanding proper etiquette and safety precautions, is of upmost importance to becoming comfortable in the operating room environment. This chapter helps familiarize new NIOM staff to the operating room, thereby allowing for a successful and safe monitoring procedure. NIOM equipment that will enter the sterile field must be properly sterilized before being brought into the operating room. There are many people, such as surgical team, anesthesiology team, nursing team, and room attendant, present in the operating room, and the chapter discusses each person’s roles and how their roles relate to NIOM. Performing NIOM in the operating room requires additional knowledge beyond evoked potentials (EP), electroencephalography (EEG) and electromyography (EMG).