Viral conjunctivitis, an infection of conjunctiva, is the most common form of conjunctivitis. Human adenoviruses (HAdV) are responsible for around 65% to 90% of all cases of viral conjunctivitis. HAdV is a non-enveloped double-stranded DNA virus of the genus Mastadenovirus and Adenoviridae family. HAdV causes different types of ocular infections in humans including pharyngoconjunctival fever and epidemic keratoconjunctivitis. While adenoviral conjunctivitis is highly contagious, it is self-limited, with symptoms lasting for about 2 weeks. Diagnosis of adenoviral conjunctivitis can be made based on the presenting clinical symptoms of conjunctivitis. The AdenoPlus Test is a CLIA waived test. It is a point-of-care test that detects conjunctivitis within 10 minutes. Cell culture with confirmatory immunofluorescence assay is performed, preferably prior to initiation of antibiotics, on a sample of the conjunctival discharge. A conjunctival specimen is sent to the laboratory for real-time polymerase chain reaction analysis and results are reported within 24 hours.